A partir de 2008, año de introducción de la técnica, los estudios con RNA-Seq se han multiplicados por más de 2822 veces sólo en 6 años. Estudios aplicando RNA-Seq han mostrado la magnitud, noción y complejidad del transcripotma. Keywords: HTS High Throughput Sequencing, NGS Next Generation Sequencing, RNA-Seq, transcriptome, gene expression.Įn los últimos años, la técnica RNA-Seq ha tenido un desarrollo acelerado y se ha convertido en el método de elección para el estudio y la caracterización de los transcriptomas dinámicos, aprovechando las tecnologías de secuenciación de alto rendimiento. This article describes RNA-Seq approach, technologies, methodologies, implementation, and methods done so far in characterizing and profiling transcriptomes. RNA-Seq is a cross and interdisciplinary method that interconnects biology to other scientific topics. As RNA-Seq approaches increase in speed and decrease in cost, more distinct researches are applied and become more common and accurate. Furthermore, some of the potential applications for RNA-Seq cannot be conducted by other methods and as yet are unique to RNA-Seq. RNA-Seq also contributes a more accurate gene expression and transcript isoform estimation than other methods. From 2008, as its introduction year, the relevant reports on RNA-Seq have been multiplied by more than 2822 times just in 6 years. RNA-Seq studies have shown the transcriptome magnitude, notion and complexity. In recent years, RNA-Seq has been the method of choice for profiling dynamic transcriptome taking advantage of high throughput sequencing technologies. The data produced by RNA-Seq, sequencing technologies and use of bioinformatics are exploding rapidly. RNA Sequencing (RNA-Seq) is a newly born tool that has revolutionized the post-genomic era. RNA-Seq: a glance at technologies and methodologies. 6 November 2014 Returned for revision: 10 December 2014 Accepted: 9 January 2015.Ĭitation / Citar este artículo como: Jazayeri SM, Melgarejo Muñoz LM, Romero HM. 421, Bogotá, Colombia.įor correspondence. Bogotá, Colombia.ĢDepartment of Biology, Faculty of Science, National University of Colombia (Universidad Nacional de Colombia). Corporación Centro de Investigación en Palma de Aceite (Cenipalma). Seyed Mehdi JAZAYERI 1, Luz Marina MELGAREJO MUÑOZ 2, Hernán Mauricio ROMERO 2.ġOil Palm Biology and Breeding Research Program. RNA-Seq: un vistazo sobre las tecnologías y metodologías RNA-SEQ: A GLANCE AT TECHNOLOGIES AND METHODOLOGIES

Rna sequence analysis